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TB-500 binds to actin, a structural protein inside cells, which preclinical researchers believe helps mobilize the satellite cells and fibroblasts responsible for muscle and connective-tissue regeneration
The cells were then treated in triplicate with 2012, 2020 (negative control), and Gambogic Acid (positive control) at titrating concentrations ranging from 100 M to 200 nM and allowed to incubate for 45 min in a 37 C incubator with 5% CO 2
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