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The assay mixture containing 50 mM sodium phosphate buffer (pH 7.8) and hypoxanthine 50 M and the cytosol fraction (50 uL) was incubated at 37C for 5 min

People who are heterozygous for CBS deficiency (between 1 in 70 to 1 in 200 in the general population) have normal homocysteine levels in 30% to 50% of cases

While it exists in almost every cell in the human body, it is found in its highest concentrations in the liver

But even accounting for reporting bias, the signal is significant

These elements can also lower cholesterol levels and reduce high levels of homocysteine, which are associated with increased risk of stroke, heart attack, or dementia