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Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere
Animal pharmacokinetic data for subcutaneous and intraperitoneal injection exist within the body of work from the Zagreb group and a smaller number of independent labs
NAC is a supplement form of the amino acid cysteine
Vitamin C can increase the active state of glutathione
It may be promising, but it is not yet proven.
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