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Metabolite extraction and MS analysis Metabolites were extracted from 5 10 6 20 10 6 cells by modified Folch extraction (chloroform, methanol and water, 8:4:3)

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It also provides practical and case-driven opinions and recommendations on the optimal use of GLP-1RAs by discussing important patient factors and clinical considerations that will help to identify those who are most likely to benefit from this class of agents