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The basic formula for the concentration is: Total HCG in vial / diluent volume = concentration in IU per ml For example, a 5,000 IU HCG vial mixed with 5 mL of diluent will give: 5,000 IU / 5 mL = 1,000 IU/mL The required volume of injection can then be calculated as: Prescribed dose / concentration = injection volume If the recommended dose is 250 IU: 250 IU / 1,000 IU/mL = 0.25 mL However, the Longeviters' HCG reconstitution calculator takes care of both calculations for you, so you dont have to work through a number of manual conversions yourself