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After washing in 0.1 M PB for 310 min, goat anti-mouse Alexa Fluor 488 or 594 secondary antibody (1:1000, Molecular Probes, Eugene, Oregon, USA), goat anti-rabbit Alexa Fluor 488 or 594 secondary antibody (1:1000

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Phenotypic heterogeneity in body fat distribution in patients with congenital generalized lipodystrophy caused by mutations in the AGPAT2 or seipin genes