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The culture medium was removed, and the cells were incubated with serum-free DMEM containing 10 M DCFH-DA at 37 C for 20 min
Breakthrough Peptide Healing: How BPC-157 & TB-500 Support Rapid Recovery at Iowa IV. www.iowaiv.com/blog/breakthrough-peptide-healing-how-bpc-157-tb-500-support-rapid-recovery-at-iowa-iv
The monoisotopic peak confirmed in ER scan was then used as a precursor ion in subsequent EPI scan to obtain positive product ion spectra of GSH and CN adducts detected by either PI/ER with polarity switch or NL/ER, respectively
