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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions
Lipopolysaccharide (LPS) H
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Dietary fat quality also has a significant influence, with unsaturated fats, particularly omega-3 fatty acids, exhibiting anti-inflammatory and insulin-sensitizing effects [19]
It could increase the metabolic rate of fat beta-oxidation and accelerate fat consumption in cells (mitochondria)
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