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For GCMS analysis 1 l of the sample (45 replicates) was injected with a split ratio of 20:1 on a Famewax, 30 m x 0.25 mm iD, 0.25 m df capillary column (Restek, Bad Homburg)

The extended-release formulation maintains therapeutic levels exceeding 50 pg/mL from approximately 2 weeks post-injection, complicating perioperative timing decisions [28]