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1 2 3 "Vitamin B12, folate, and the methionine remethylation cycle-biochemistry, pathways, and regulation" (PDF)

However, unlike the natural hormone, which breaks down in minutes, agonists are chemically modified to resist degradation, allowing them to remain active in the body for hours or days to provide continuous metabolic control

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After centrifugation, cell pellets were then collected and resuspended with stimulation buffer [HBSS supplemented with 5 mM HEPES, 0.5 mM IBMX and 0.1% (w/v) BSA, pH 7.4] to a density of 0.6 million cells per mL and added to 384-well culture plates (3,000 cells per well)

7th International Congress of Endocrinology, Quebec, Canada, 1984, p