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The complexity of producing GLP-1 drugs, which often come in injectable pen formats, has added further delays
Common requirements include documented BMI meeting the threshold, proof of previous weight loss attempts (usually 3-6 months of supervised diet and exercise), at least one qualifying comorbidity, and sometimes evidence that other treatments have been tried and failed
Wash with PBS one more time and add 500 l of lysis buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5) on ice
One analysis used medical and pharmacy claims data to evaluate outcomes in 371,698 patients with type 2 diabetes initiating GLP-1 RA, SGLT2i, DPP-4i and sulfonylurea class medications, with the primary outcome being time-to-treatment for DME or PDR
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