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As a consequence, a loss of lipoic acid-dependent enzymatic activity caused by defects in endogenous lipoic acid synthesis (see Deficiency) cannot be rescued by the provision of exogenous lipoic acid (5)

The resulting solution was purified using EMD Millipore Amicon Ultra-0.5 centrifugal filter units with a membrane molecular weight cut-off (MWCO) of 10 kDa were used to remove residual ions (i.e