The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea
Peptides are naturally occurring amino acid chains that help regulate important processes like metabolism, tissue repair, energy, and cellular signaling
Wei H, Saladi R, Lu Y, et al
Regardless of the method, sterile technique, proper needle size, and careful site rotation (for in vivo studies) are paramount
The pQCT data perhaps suggests that the rise in cortical BMC may be attributed to a growth in the cross-sectional bone area, even though the cortical volumetric BMD seemingly stayed the same