The primary antibodies used are as follows: P53 (Proteintech, 60283-2-Ig), pSer46-P53 (CST, 2521), FOXO4 (Proteintech, 21535-1-AP), Ki-67 (D3B5) Rabbit mAb (Alexa Fluor 647 Conjugate) (CST, 12075), P21 (CST, 14074), -H2AX (Abcam, ab2254)
Click on a question to see more A shot is injected into the muscle with a syringe, which can be done in les than five minutes, however does not provide hydration
Specific dosing parameters vary significantly between studies and animal models researchers should reference the primary literature for protocol design
Salem et al., 2015) (Figure 4)
Yet, the sentiment to just let people decide is growing, even among people working in traditional healthcare