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The soluble fraction was clarified by centrifugation (75,000 g , 20 min, 4 C) and incubated with 50% Ni-Sepharose suspension (GE Healthcare, Chalfont St Giles, UK) for 2 h at 4 C with gentle rotation prior to affinity purification
prausnitzii is particularly at risk, as this bacterium is largely dependent upon adequate dietary consumption of fermentable soluble fiber, found in legumes, fruits, vegetables, and whole grains
51 HF hospitalisations were significantly reduced in patients with a preserved, but not reduced, ejection fraction
injections of 100 nmol kg 1 MK-801, 100 nmol kg 1 GLP-1, 100 nmol kg 1 GLP-1MK-801 or vehicle (isotonic saline) for 3 days
PMC 2739594
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