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Experiments in Isolated Heart Preparation 4 1.19 mmol/L, NaHCO 3 25 mmol/L, KH 2 PO 4 1.19 mmol/L, d-glucose 5 mmol/L, CaCl 2 1.4 mmol/L, and pyruvate 2 mmol/L), cannulated via the aorta, and mounted onto a Langendorff apparatus to establish retrograde perfusion via the ascending aorta with oxygenated Krebs solution at a constant flow of 2 mL/min

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During a hiccup, the diaphragm suddenly contracts and pulls down between breaths, then the vocal cords snap shut briefly, producing the characteristic hic noise

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