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Quantitative real-time PCR (qRT-PCR) of mRNA and microbial DNA Total RNA from tissues was extracted using TRIzol reagent (Yamei, Shanghai, China) and reverse-transcribed into complementary DNA (cDNA) with a reverse transcription kit (Vazyme, Nanjing, China)
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To obtain complete read counts for 3 and 5 miRNA species, the mapping output was parsed
Epub 2011 Apr 14
An endogeous defensive concept, renewed cytoprotection/adaptive cytoprotection: intra(per)-oral/intragastric strong alcohol in rat
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