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These AG-haESCs were cultured in 2i medium consisting of DMEM (Millipore, Billerica, MA, USA) supplemented with 15% FBS (ExCell Bio, Shanghai, China), penicillinstreptomycin, non-essential amino acids, nucleosides, L-glutamine, -mercaptoethanol, 1000 U/mL LIF, 1 M PD0325901 (Selleck Chemicals, Houston, TX, USA), and 3 M CHIR99021 (Selleck Chemicals)

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2015;15:e4.